Publications

Bachand, George B.; Bachand, Marlene B.; Greene, Adrienne C.; Stevens, Mark J.

Abstract not provided.

Sasaki, Darryl Y.; Bachand, George B.; Spoerke, Erik D.; Stevens, Mark J.; Vandelinder, Virginia A.; Martinez, Haneen M.; Bachand, Marlene B.; Greene, Adrienne C.; Nabar, Namita N.; Paxton, Walter F.

Abstract not provided.

Chemical Communications

Greene, A.C.; Bachand, Marlene B.; Gomez, A.; Stevens, Mark J.; Bachand, George B.

The directed, head-to-tail self-assembly of microtubule filaments may be generalized in the context of Janus colloidal rods. Specifically, their assembly at the tens of micron-length scale involves a careful balance between long-range electrostatic repulsion and short-range attractive forces. Here we show that the addition of counterion salts increases the rate of directed assembly by screening the electrostatic forces and enhancing the effectiveness of short-range interactions at the microtubule ends.

Greene, Adrienne C.; Bachand, Marlene B.; Bachand, George B.; Henderson, Ian M.; Paxton, Walter F.; Vandelinder, Virginia A.; gomez, andrew g.

Abstract not provided.

Greene, Adrienne C.; Bachand, George B.; Bachand, Marlene B.; gomez, andrew g.; Henderson, Ian M.

Abstract not provided.

Greene, Adrienne C.; Bachand, Marlene B.; Bachand, George B.; gomez, andrew g.

Abstract not provided.

Bachand, George B.; Bachand, Marlene B.; Greene, Adrienne C.; gomez, andrew g.

Abstract not provided.

Greene, Adrienne C.; Bachand, Marlene B.; gomez, andrew g.; Bachand, George B.

Abstract not provided.

Greene, Adrienne C.; gomez, andrew g.; Bachand, George B.; Bachand, Marlene B.; Henderson, Ian M.

Abstract not provided.

RSC Advances

Bachand, Marlene B.; Bouxsein, Nathan F.; Cheng, S.; Von Hoyningen-Huene, S.J.; Stevens, Mark J.; Bachand, George B.

Microtubules (MTs) are biological polymer filaments that display unique polymerization dynamics, and serve as inspiration for developing synthetic nanomaterials that exhibit similar assembly-derived behaviours. Here we explore an assembly process in which extended 1D nano-arrays (NAs) are formed through the directed, head-to-tail self-assembly of MT filaments. In particular, we demonstrate that the elongation of NAs over time is due to directed self-assembly of MTs by a process that is limited by diffusion and follows second-order rate kinetics. We further described a mechanism, both experimental and through molecular dynamics simulations, where stable junctions among MT building blocks are formed by alignment and adhesion of opposing filament ends, which is followed by formation of a stable junction through the incorporation of free tubulin and the removal of lattice vacancies. The fundamental principles described in this directed self-assembly process provide a promising basis for new approaches to manufacturing complex, heterostructured nanocomposites.

Bachand, George B.; Bachand, Marlene B.; Bouxsein, Nathan F.; Sasaki, Darryl Y.; von Hoyningen-Huene, Sergei J.

Abstract not provided.

WIREs Nanomedicine and Nanobiotechnology

Bachand, George B.; Bouxsein, Nathan F.; Vandelinder, Virginia A.; Bachand, Marlene B.

Abstract not provided.

Sasaki, Darryl Y.; Bouxsein, Nathan F.; Bachand, Marlene B.; Bachand, George B.

Abstract not provided.

Bachand, Marlene B.; von Hoyningen-Huene, Sergei J.; Cheng, Shengfeng C.; Bouxsein, Nathan F.; Spoerke, Erik D.; Bachand, George B.

Abstract not provided.

Bachand, George B.; Brozik, Susan M.; Bachand, Marlene B.; Aaron, Jesse S.; Timlin, Jerilyn A.; Achyuthan, Komandoor A.; Kotula, Paul G.

Engineered nanomaterials (ENMs) are increasingly being used in commercial products, particularly in the biomedical, cosmetic, and clothing industries. For example, pants and shirts are routinely manufactured with silver nanoparticles to render them 'wrinkle-free.' Despite the growing applications, the associated environmental health and safety (EHS) impacts are completely unknown. The significance of this problem became pervasive within the general public when Prince Charles authored an article in 2004 warning of the potential social, ethical, health, and environmental issues connected to nanotechnology. The EHS concerns, however, continued to receive relatively little consideration from federal agencies as compared with large investments in basic nanoscience R&D. The mounting literature regarding the toxicology of ENMs (e.g., the ability of inhaled nanoparticles to cross the blood-brain barrier; Kwon et al., 2008, J. Occup. Health 50, 1) has spurred a recent realization within the NNI and other federal agencies that the EHS impacts related to nanotechnology must be addressed now. In our study we proposed to address critical aspects of this problem by developing primary correlations between nanoparticle properties and their effects on cell health and toxicity. A critical challenge embodied within this problem arises from the ability to synthesize nanoparticles with a wide array of physical properties (e.g., size, shape, composition, surface chemistry, etc.), which in turn creates an immense, multidimensional problem in assessing toxicological effects. In this work we first investigated varying sizes of quantum dots (Qdots) and their ability to cross cell membranes based on their aspect ratio utilizing hyperspectral confocal fluorescence microscopy. We then studied toxicity of epithelial cell lines that were exposed to different sized gold and silver nanoparticles using advanced imaging techniques, biochemical analyses, and optical and mass spectrometry methods. Finally we evaluated a new assay to measure transglutaminase (TG) activity; a potential marker for cell toxicity.

Bachand, George B.; Bachand, Marlene B.; von Hoyningen-Huene, Sergei J.; Cheng, Shengfeng C.; Stevens, Mark J.; Bouxsein, Nathan F.; Spoerke, Erik D.; Bunker, B.C.

Abstract not provided.

Bachand, George B.; Bachand, Marlene B.; Brozik, Susan M.; Achyuthan, Komandoor A.

Abstract not provided.

Biophysical Journal

Schwarz, Haiqing L.; Bachand, Marlene B.; Stachowiak, Jeanne C.; Hayden, Carl C.; Bunker, B.C.; Bachand, George B.

Abstract not provided.