Publications

Martinez, Haneen M.; Rush, Matthew N.; Guo, Jimin G.; Brinker, C.J.; Bachand, George B.

Abstract not provided.

Ko, Randy F.; Jain, Rishi J.; Bouxsein, Nathan F.; Vandelinder, Virginia A.; Bachand, George B.

Abstract not provided.

Paxton, Walter F.; Howell, Stephen W.; Moorman, Matthew W.; Manginell, Ronald P.; Bachand, George B.; Achyuthan, Komandoor A.

Abstract not provided.

Vandelinder, Virginia A.; Celleti, Giorgia C.; Lemke, Edward L.; Bachand, George B.

Abstract not provided.

Sasaki, Darryl Y.; Bachand, George B.; Spoerke, Erik D.; Stevens, Mark J.; Vandelinder, Virginia A.; Martinez, Haneen M.; Bachand, Marlene B.; Greene, Adrienne C.; Nabar, Namita N.; Paxton, Walter F.

Abstract not provided.

Spoerke, Erik D.; Bachand, George B.; Sasaki, Darryl Y.; Stevens, Mark J.; Bollinger, Jonathan B.; Jones, Brad H.; Wheeler, Jill S.; Vervacke, Jeffrey V.; Martinez, Alina M.; mcgrath, dominic m.

Abstract not provided.

Spoerke, Erik D.; Jones, Brad H.; Martinez, Alina M.; Small, Leo J.; Wheeler, David R.; Wheeler, Jill S.; Bachand, George B.

Abstract not provided.

Bachand, George B.

Abstract not provided.

Bachand, George B.; Vandelinder, Virginia A.; Ting, Christina T.; Brener, Stephanie B.

Abstract not provided.

Vandelinder, Virginia A.; Adams, Peter A.; Bachand, George B.

Abstract not provided.

Chemical Communications

Greene, A.C.; Bachand, Marlene B.; Gomez, A.; Stevens, Mark J.; Bachand, George B.

The directed, head-to-tail self-assembly of microtubule filaments may be generalized in the context of Janus colloidal rods. Specifically, their assembly at the tens of micron-length scale involves a careful balance between long-range electrostatic repulsion and short-range attractive forces. Here we show that the addition of counterion salts increases the rate of directed assembly by screening the electrostatic forces and enhancing the effectiveness of short-range interactions at the microtubule ends.

Scientific Reports

Vandelinder, Virginia A.; Adams, Peter G.; Bachand, George B.

The fundamental biophysics of gliding microtubule (MT) motility by surface-tethered kinesin-1 motor proteins has been widely studied, as well as applied to capture and transport analytes in bioanalytical microdevices. In these systems, phenomena such as molecular wear and fracture into shorter MTs have been reported due the mechanical forces applied on the MT during transport. In the present work, we show that MTs can be split longitudinally into protofilament bundles (PFBs) by the work performed by surface-bound kinesin motors. We examine the properties of these PFBs using several techniques (e.g., fluorescence microscopy, SEM, AFM), and show that the PFBs continue to be mobile on the surface and display very high curvature compared to MT. Further, higher surface density of kinesin motors and shorter kinesin-surface tethers promote PFB formation, whereas modifying MT with GMPCPP or higher paclitaxel concentrations did not affect PFB formation.

Langmuir

Bordovsky, Stefan S.; Wong, Christopher S.; Bachand, George B.; Stachowiak, Jeanne C.; Sasaki, Darryl Y.

The selective partitioning of lipid components in phase-separated membranes is essential for domain formation involved in cellular processes. Identifying and tracking the movement of lipids in cellular systems would be improved if we understood how to achieve selective affinity between fluorophore-labeled lipids and membrane assemblies. Here, we investigated the structure and chemistry of membrane lipids to evaluate lipid designs that partition to the liquid ordered (Lo) phase. A range of fluorophores at the headgroup position and lengths of PEG spacer between the lipid backbone and fluorophore were examined. On a lipid body with saturated palmityl or palmitoyl tails, we found that although the lipid tails can direct selective partitioning to the Lo phase through favorable packing interactions, headgroup hydrophobicity can override the partitioning behavior and direct the lipid to the disordered membrane phase (Ld). The PEG spacer can serve as a buffer to mute headgroup-membrane interactions and thus improve Lo phase partitioning, but its effect is limited with strongly hydrophobic fluorophore headgroups. We present a series of lipid designs leading to the development of novel fluorescently labeled lipids with selective affinity for the Lo phase.

Ruffing, Anne R.; Bachand, George B.; Timlin, Jerilyn A.; Manginell, Ronald P.; Hudson, Corey H.; Williams, Kelly P.; Rempe, Susan R.; Brinker, C.J.; Olszewska-Wasiolek, Maryla A.; Hanson, Donald J.; VanderNoot, Victoria A.

Abstract not provided.

PLoS ONE

Greene, Adrienne C.; Henderson, Ian M.; Gomez, Andrew; Paxton, Walter F.; Vandelinder, Virginia A.; Bachand, George B.

Polymersomes are being widely explored as synthetic analogs of lipid vesicles based on their enhanced stability and potential uses in a wide variety of applications in (e.g., drug delivery, cell analogs, etc.). Controlled formation of giant polymersomes for use in membrane studies and cell mimetic systems, however, is currently limited by low-yield production methodologies. Here, we describe for the first time, how the size distribution of giant poly(ethylene glycol)-poly(butadiene) (PEO-PBD) polymersomes formed by gel-assisted rehydration may be controlled based on membrane fluidization. We first show that the average diameter and size distribution of PEO-PBD polymersomes may be readily increased by increasing the temperature of the rehydration solution. Further, we describe a correlative relationship between polymersome size and membrane fluidization through the addition of sucrose during rehydration, enabling the formation of PEO-PBD polymersomes with a range of diameters, including giant-sized vesicles (>100 μm). This correlative relationship suggests that sucrose may function as a small molecule fluidizer during rehydration, enhancing polymer diffusivity during formation and increasing polymersome size. Overall the ability to easily regulate the size of PEO-PBD polymersomes based on membrane fluidity, either through temperature or fluidizers, has broadly applicability in areas including targeted therapeutic delivery and synthetic biology.

Journal of Visualized Experiments

Greene, Adrienne C.; Sasaki, Darryl Y.; Bachand, George B.

Polymer vesicles, or polymersomes, are being widely explored as synthetic analogs of lipid vesicles based on their stability, robustness, barrier properties, chemical versatility and tunable physical characteristics. Typical methods used to prepare giant-sized (> 4 μm) vesicles, however, are both time and labor intensive, yielding low numbers of intact polymersomes. Here, we present for the first time the use of gel-assisted rehydration for the rapid and high-yielding formation of giant (>4 μm) polymer vesicles (polymersomes). Using this method, polymersomes can be formed from a wide array of rehydration solutions including several different physiologically-compatible buffers and full cell culture media, making them readily useful for biomimicry studies. This technique is also capable of reliably producing polymersomes from different polymer compositions with far better yields and much less difficulty than traditional methods. Polymersome size is readily tunable by altering temperature during rehydration or adding membrane fluidizers to the polymer membrane, generating giant-sized polymersomes (>100 μm).

Nanoscale

Mahajan, K.D.; Ruan, G.; Dorcéna, C.J.; Vieira, G.; Nabar, G.; Bouxsein, N.F.; Chalmers, J.J.; Bachand, George B.; Sooryakumar, R.; Winter, J.O.

Nanoscale control of matter is critical to the design of integrated nanosystems. Here, we describe a method to dynamically control directionality of microtubule (MT) motion using programmable magnetic fields. MTs are combined with magnetic quantum dots (i.e., MagDots) that are manipulated by external magnetic fields provided by magnetic nanowires. MT shuttles thus undergo both ATP-driven and externally-directed motion with a fluorescence component that permits simultaneous visualization of shuttle motion. This technology is used to alter the trajectory of MTs in motion and to pin MT motion. Such an approach could be used to evaluate the MT-kinesin transport system and could serve as the basis for improved lab-on-a-chip technologies based on MT transport.

Bachand, George B.

Abstract not provided.

Bachand, George B.

Abstract not provided.

Greene, Adrienne C.; Bachand, Marlene B.; Bachand, George B.; Henderson, Ian M.; Paxton, Walter F.; Vandelinder, Virginia A.; gomez, andrew g.

Abstract not provided.

Bachand, George B.; Vandelinder, Virginia A.; Brener, Stephanie B.

Abstract not provided.

Spoerke, Erik D.; Jones, Brad H.; Wheeler, Jill S.; Martinez, Alina M.; Ting, Christina T.; Stevens, Mark J.; Bachand, George B.

Abstract not provided.

Spoerke, Erik D.; Jones, Brad H.; Martinez, Alina M.; Wheeler, Jill S.; Wheeler, David R.; Ting, Christina T.; Stevens, Mark J.; Bachand, George B.

Abstract not provided.

Greene, Adrienne C.; Bachand, George B.; Bachand, Marlene B.; gomez, andrew g.; Henderson, Ian M.

Abstract not provided.

Biomacromolecules

Vandelinder, Virginia A.; Brener, Stephanie; Bachand, George B.

Active self-assembly offers a powerful route for the creation of dynamic multiscale structures that are presently inaccessible with standard microfabrication techniques. One such system uses the translation of microtubule filaments by surface-tethered kinesin to actively assemble nanocomposites with bundle, ring, and spool morphologies. Attempts to observe mechanisms involved in this active assembly system have been hampered by experimental difficulties with performing observation during buffer exchange and photodamage from fluorescent excitation. In the present work, we used a custom microfluidic device to remove these limitations and directly study ring/spool formation, including the earliest events (nucleation) that drive subsequent nanocomposite assembly. Three distinct formation events were observed: pinning, collisions, and induced curvature. Of these three, collisions accounted for the majority of event leading to ring/spool formation, while the rate of pinning was shown to be dependent on the amount of photodamage in the system. We further showed that formation mechanism directly affects the diameter and rotation direction of the resultant rings and spools. Overall, the fundamental understanding described in this work provides a foundation by which the properties of motor-driven, actively assembled nanocomposites may be tailored toward specific applications.