Publications

Iwai, Kosuke; sustarich, J.s.; Kim, Peter W.; Gach, Philip C.; Raje, Manasi R.; Heinemann, J.V.H.; Duncombe, Todd A.; Deng, Kai; Northen, T.R.N.; Hillson, N.J.H.; Adams, P.D.A.; Singh, Anup K.

Abstract not provided.

Lab on a Chip

Gach, Philip C.; Iwai, Kosuke; Kim, Peter W.; Hillson, Nathan J.; Singh, Anup K.

Synthetic biology is an interdisciplinary field that aims to engineer biological systems for useful purposes. Organism engineering often requires the optimization of individual genes and/or entire biological pathways (consisting of multiple genes). Advances in DNA sequencing and synthesis have recently begun to enable the possibility of evaluating thousands of gene variants and hundreds of thousands of gene combinations. However, such large-scale optimization experiments remain cost-prohibitive to researchers following traditional molecular biology practices, which are frequently labor-intensive and suffer from poor reproducibility. Liquid handling robotics may reduce labor and improve reproducibility, but are themselves expensive and thus inaccessible to most researchers. Microfluidic platforms offer a lower entry price point alternative to robotics, and maintain high throughput and reproducibility while further reducing operating costs through diminished reagent volume requirements. Droplet microfluidics have shown exceptional promise for synthetic biology experiments, including DNA assembly, transformation/transfection, culturing, cell sorting, phenotypic assays, artificial cells and genetic circuits.

Gach, Philip C.

Abstract not provided.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Gach, Philip C.; Shih, Steve C.C.; Sustarich, Jess; Hillson, Nathan J.; Adams, Paul D.; Singh, Anup K.

Synthetic biology experiments require optimization of pathways consisting of many genes and other genetic elements and given the large number of alternatives available for each element, optimization of a pathway can require large number of experiments consuming prohibitively-expensive amounts of DNA and enzymes. Digital microfluidics (DMF), because of its ability to process small volumes, presents a cost-effective solution for conducting high-throughput cloning and expression experiments. We describe the first DMF device for automating all critical steps of transformation and culture including plasmid addition, transformation by heat-shock, addition of selection medium, culture and expression of GFP.