Meeting User Needs: Developing Field-Deployable Biodetection Systems Using a Micro-Separations Approach
Abstract not provided.
Publications
Search for Anthrax Biomarkers in Mouse Serum using a Mesofluidic Automated Processing Platform
Abstract not provided.
Modular Automated Processing System (MAPS) for High-Throughput Automated Serum Processing
Abstract not provided.
MODULAR DESIGN FOR AUTOMATED PROCESSING OF COMPLEX BIOLOGICAL SAMPLES
Abstract not provided.
Copy of High Throughput Methods To Identify Underlying Molecular Signature Pathways In Desulfovibrio vulgaris
Abstract not provided.
A Survey of Protein Post-Translational Modifications Found in the Sulfate-Reducing Bacterium Desulfovibrio vulgaris Hildenborough
Abstract not provided.
Size exclusion chromatography of microliter volumes for on-line use in low-pressure microfluidic systems
Analytical Chemistry
We have developed a new cartridge format for on-line size exclusion processing in low-pressure, portable microfluidic devices. The described system allows size exclusion chromatography of microliter volumes (termed μL-SEC) to be performed with ready integration in complex protocols for continuous-flow sample processing and analysis. The refillable cartridge format was employed for the preparation of Bacillus subtilus spores lysed in the presence of a strong reducing agent. While the reducing agent is known to interfere with subsequent fluorescent labeling of the solubilized proteins, the described continuous-flow size exclusion processing allowed complete isolation of interferences from a 10-μL sample in 70 s. Following efficient labeling, the protein sample was injected and separated on-chip using gel electrophoresis. To increase the resolution, speed, and sample capacity of buffer exchange under low-pressure operation (40 psi), parameters such as the size exclusion resin, load volumes, flow rates, buffer composition, and cartridge geometry were optimized and are presented here. The μL-SEC analysis is compatible with automated sample preparation for microfluidic systems and has resulted in significantly increased analysis speed and throughput over benchtop methods. The presented technique has the potential to improve capabilities such as buffer exchange, size fractionation, and high-abundance protein removal-steps that are frequently required prior to on-chip, point-of-care, and mass spectrometric analyses. © 2006 American Chemical Society.
Modular Automated Processing System (MAPS) for Bioanalysis
Abstract not provided.
Development of modules for multidimensional on-line processing of microliter volume samples
Abstract not provided.
Modular design for automated processing of complex biological samples
Abstract not provided.
Integrated Sample Porcessing System for Fully Autonomous Operation of the uChemLab Biodetector
Abstract not provided.
Novel approaches to microliter volume SPE for liquid sample preparation
Abstract not provided.
Analysis of peptides using an integrated microchip HPLC-MS/MS system
Hyphendated LC-MS techniques are quickly becoming the standard tool for protemic analyses. For large homogeneous samples, bulk processing methods and capillary injection and separation techniques are suitable. However, for analysis of small or heterogeneous samples, techniques that can manipulate picoliter samples without dilution are required or samples will be lost or corrupted; further, static nanospray-type flowrates are required to maximize SNR. Microchip-level integration of sample injection with separation and mass spectrometry allow small-volume analytes to be processed on chip and immediately injected without dilution for analysis. An on-chip HPLC was fabricated using in situ polymerization of both fixed and mobile polymer monoliths. Integration of the chip with a nanospray MS emitter enables identification of peptides by the use of tandem MS. The chip is capable of analyzing of very small sample volumes (< 200 pl) in short times (< 3 min).
13 Results