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Differential white cell count by centrifugal microfluidics

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Schaff, U.Y.; Tentori, A.M.; Sommer, G.J.

We present a method for counting white blood cells that is uniquely compatible with centrifugation based microfluidics. Blood is deposited on top of one or more layers of density media within a microfluidic disk. Spinning the disk causes the cell populations within whole blood to settle through the media, reaching an equilibrium based on the density of each cell type. Separation and fluorescence measurement of cell types stained with a DNA dye is demonstrated using this technique. The integrated signal from bands of fluorescent microspheres is shown to be proportional to their initial concentration in suspension.

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TYPE Conference YEAR 2010
ScopusOSTI

Microscale isoelectric fractionation using immobilized ph-specific membranes for multi-dimensional analysis

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Mai, Junyu M.; Sommer, G.J.; Hatch, Anson H.

We report on advancements of our microscale isoelectric fractionation (μIEFr) methodology for fast on-chip separation and concentration of proteins based on their isoelectric points (pI). We establish that proteins can be fractionated depending on posttranslational modifications into different pH specific bins, from where they can be efficiently transferred to downstream membranes for additional processing and analysis. This technology can enable on-chip multidimensional glycoproteomics analysis, as a new approach to expedite biomarker identification and verification.

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TYPE Conference YEAR 2010
ScopusOSTI

Microscale isoelectric fractionation using immobilized pH-specific membranes for multi-dimensional analysis

Sommer, G.J.; Hatch, Anson H.

We report on advancements of our microscale isoelectric fractionation ({mu}IEFr) methodology for fast on-chip separation and concentration of proteins based on their isoelectric points (pI). We establish that proteins can be fractionated depending on posttranslational modifications into different pH specific bins, from where they can be efficiently transferred to downstream membranes for additional processing and analysis. This technology can enable on-chip multidimensional glycoproteomics analysis, as a new approach to expedite biomarker identification and verification.

More Details
TYPE Conference YEAR 2010
OSTI
Results 1–25 of 26
Results 1–25 of 26