Patch-clamp and live fluorescence imaging analysis of the effect of potassium channel blockade on lipopolysaccharide-induced immune signaling
Abstract not provided.
Publications
Characterization of the TLR4-Mediated Response to Y. pestis and E. coli LPS in Murine Macrophages
Abstract not provided.
Transcription Factor Translocation in Response to Different Biothreat Agents LPS Chemotypes in Macrophages
Abstract not provided.
Copy of Transcription Factor Translocation in Response to Different Biothreat Agents LPS Chemotypes in Macrophages
Abstract not provided.
Luminescence Investigations of Metal Transport and Antimicrobial Peptide (AMP) Activities
Luminescence
Abstract not provided.
An abridged salting-out method for isolation of high-quality genomic DNA
Proposed for publication in Bio Techniques.
Abstract not provided.
Integration of biological ion channels onto optically addressable micro-fluidic electrode arrays for single molecule characterization
The challenge of modeling the organization and function of biological membranes on a solid support has received considerable attention in recent years, primarily driven by potential applications in biosensor design. Affinity-based biosensors show great promise for extremely sensitive detection of BW agents and toxins. Receptor molecules have been successfully incorporated into phospholipid bilayers supported on sensing platforms. However, a collective body of data detailing a mechanistic understanding of membrane processes involved in receptor-substrate interactions and the competition between localized perturbations and delocalized responses resulting in reorganization of transmembrane protein structure, has yet to be produced. This report describes a systematic procedure to develop detailed correlation between (recognition-induced) protein restructuring and function of a ligand gated ion channel by combining single molecule fluorescence spectroscopy and single channel current recordings. This document is divided into three sections: (1) reported are the thermodynamics and diffusion properties of gramicidin using single molecule fluorescence imaging and (2) preliminary work on the 5HT{sub 3} serotonin receptor. Thirdly, we describe the design and fabrication of a miniaturized platform using the concepts of these two technologies (spectroscopic and single channel electrochemical techniques) for single molecule analysis, with a longer term goal of using the physical and electronic changes caused by a specific molecular recognition event as a transduction pathway in affinity based biosensors for biotoxin detection.
7 Results