Publications

Hayes, Dulce C.

Abstract not provided.

Ye, Dongmei Y.; Hayes, Dulce C.; Maes, Danae M.; Simoes, Casey T.; Appelhans, Leah A.; Kent, Michael S.; Timlin, Jerilyn A.

Abstract not provided.

Dailey, Meghan C.; Ye, Dongmei Y.; Sale, Kenneth L.; Maes, Danae M.; Simoes, Casey T.; Appelhans, Leah A.; Hayes, Dulce C.; Kent, Michael S.; Timlin, Jerilyn A.

Abstract not provided.

Dailey, Meghan C.; Ye, Dongmei Y.; Maes, Danae M.; Simoes, Casey T.; Appelhans, Leah A.; Hayes, Dulce C.; Kent, Michael S.; Timlin, Jerilyn A.

Abstract not provided.

Dailey, Meghan C.; Hayes, Dulce C.; Ye, Dongmei Y.; Simoes, Casey T.; Appelhans, Leah A.; Maes, Danae M.; Kent, Michael S.; Timlin, Jerilyn A.

Abstract not provided.

Kent, Michael S.; Xiao, Xiaoyin; Rader, Nadeya C.; Simoes, Casey T.; Appelhans, Leah A.; Carson, Bryan C.; Hayes, Dulce C.; Timlin, Jerilyn A.

Abstract not provided.

Kent, Michael S.; Dailey, Meghan C.; Ye, Dongmei Y.; Simoes, Casey T.; Appelhans, Leah A.; Hayes, Dulce C.; Kent, Michael S.; Timlin, Jerilyn A.

Abstract not provided.

Rapid communications in mass spectrometry : RCM

Xiao, Xiaoyin; Miller, Lance L.; Parchert, Kylea J.; Hayes, Dulce C.; Hochrein, James M.

RATIONALE: From allergies to plant reproduction, pollens have important impacts on the health of human and plant populations, yet identification of pollen grains remains difficult and time-consuming. Low-volatility flavonoids generated from pollens cannot be easily characterized and quantified with current analytical techniques. METHODS: Here we present the novel use of atmospheric solids analysis probe mass spectrometry (ASAP-MS) for the characterization of flavonoids in pollens. Flavonoid patterns were generated for pollens collected from different plant types (trees and bushes) in addition to bee pollens from distinct geographic regions. Standard flavonoids (kaempferol and rhamnazin) and those produced from pollens were compared and assessed with ASAP-MS using low-energy collision MS/MS. Results for a semi-quantitative method for assessing the amount of a flavonoid in pollens are also presented. RESULTS: Flavonoid patterns for pollen samples were distinct with variability in the number and relative abundance of flavonoids in each sample. Pollens contained 2-5 flavonoids, and all but Kochia scoparia contained kaempferol or kaempferol isomers. We establish this method as a reliable and applicable technique for analyzing low-volatility compounds with minimal sample preparation. Standard curves were generated using 0.2-5 μg of kaempferol; from these experiments, it was estimated that there is approximately 2 mg of kaempferol present in 1 g of P. nigra italica pollen. CONCLUSIONS: Pollens can be characterized with a simple flavonoid pattern rather than analyzing the whole product pattern or the products-temperature profiles. ASAP-MS is a rapid analytical technique that can be used to distinguish between plant pollens and between bee pollens originating from different regions. Copyright © 2016 John Wiley & Sons, Ltd.

Polsky, Ronen P.; Appelhans, Leah A.; Wheeler, David R.; Jungjohann, Katherine L.; Hayes, Dulce C.; Campbell, DeAnna M.; Rudolph, Angela R.; Rivas, Rhiana R.; Zubelewicz, Michael C.; Shreve, Andrew S.; Graves, Steve G.; Brozik, Susan M.

Optical fluorescence-based DNA assays are commonly used for pathogen detection and consist of an optical substrate containing DNA capture molecules, binding of target RNA or DNA sequences, followed by detection of the hybridization event with a fluorescent probe. Though fluorescence detection can offer exquisite signal-to-background ratios, with high specificity, vast opportunities exist to improve current optical-based genomic sensing approaches. For these reasons, there is a clear need to explore alternative optical sensing paradigms to alleviate these restrictions. Bio-templated nanomaterial synthesis has become a powerful concept for developing new platforms for bio-sensing, as the biomolecule of interest can act as part of the sensing transducer mechanism. We explored the use of DNA origami structures to immobilize gold nanoparticles in very precise localized arrangements producing unique optical absorption properties with implications in novel DNA sensing schemes. We also explored the use of in-situ TEM as a novel characterization method for DNA-nanoparticle assemblies.

Xiao, Xiaoyin; Hochrein, James M.; Miller, Lance L.; Parchert, Kylea J.; Hayes, Dulce C.

Abstract not provided.

Haworth, Jacob J.; Brozik, Susan M.; Parchert, Kylea J.; Hayes, Dulce C.

Abstract not provided.

Grillet, Anne M.; Reichert, Matthew D.; Brotherton, Christopher M.; Koehler, Timothy P.; Harper, Jason C.; Hayes, Dulce C.; Brinker, C.J.; Brozik, Susan M.

Abstract not provided.

Davis, Ryan W.; Hayes, Dulce C.; Polsky, Ronen P.; Brozik, Susan M.

Formation of complex nanomaterials would ideally involve single-pot reaction conditions with one reactive site per nanoparticle, resulting in a high yield of incrementally modified or oriented structures. Many studies in nanoparticle functionalization have sought to generate highly uniform nanoparticles with tailorable surface chemistry necessary to produce such conjugates, with limited success. In order to overcome these limitations, we have modified commercially available nanoparticles with multiple potential reaction sites for conjugation with single ssDNAs, proteins, and small unilamellar vesicles. These approaches combined heterobifunctional and biochemical template chemistries with single molecule optical methods for improved control of nanomaterial functionalization. Several interesting analytical results have been achieved by leveraging techniques unique to SNL, and provide multiple paths for future improvements for multiplex nanoparticle synthesis and characterization. Hyperspectral imaging has proven especially useful for assaying substrate immobilized fluorescent particles. In dynamic environments, temporal correlation spectroscopies have been employed for tracking changes in diffusion/hydrodynamic radii, particle size distributions, and identifying mobile versus immobile sample fractions at unbounded dilution. Finally, Raman fingerprinting of biological conjugates has been enabled by resonant signal enhancement provided by intimate interactions with nanoparticles and composite nanoshells.

Proposed for publication in Langmuir.

Hayes, Dulce C.; Jones, Howland D.; Van Benthem, Mark V.; Brozik, Susan M.; Sinclair, Michael B.

Abstract not provided.

Luminescence

Hayes, Dulce C.; Carles, Elizabeth L.; Meyer, Lauren A.; Brozik, Susan M.

Abstract not provided.

Proposed for publication in Bio Techniques.

Hayes, Dulce C.; Carles, Elizabeth L.; Brozik, Susan M.; Dolan, Patricia L.

Abstract not provided.

Hayes, Dulce C.; Dolan, Patricia L.; Harper, Jason C.; Brozik, Susan M.

Chemical or biological sensors that are specific, sensitive, and robust allowing intelligence gathering for verification of nuclear non-proliferation treaty compliance and detouring production of weapons of mass destruction are sorely needed. Although much progress has been made in the area of biosensors, improvements in sensor lifetime, robustness, and device packaging are required before these devices become widely used. Current chemical and biological detection and identification techniques require less-than-covert sample collection followed by transport to a laboratory for analysis. In addition to being expensive and time consuming, results can often be inconclusive due to compromised sample integrity during collection and transport. We report here a demonstration of a plant based sensor technology which utilizes mature and seedling plants as chemical sensors. One can envision genetically modifying native plants at a site of interest that can report the presence of specific toxins or chemicals. In this one year project we used a developed inducible expression system to show the feasibility of plant sensors. The vector was designed as a safe, non-infectious vector which could be used to invade, replicate, and introduce foreign genes into mature host plants that then allow the plant to sense chem/bio agents. The genes introduced through the vector included a reporter gene that encodes for green fluorescent protein (GFP) and a gene that encodes for a mammalian receptor that recognizes a chemical agent. Specifically, GFP was induced by the presence of 17-{beta}-Estradiol (estrogen). Detection of fluorescence indicated the presence of the target chemical agent. Since the sensor is a plant, costly device packaging development or manufacturing of the sensor were not required. Additionally, the biological recognition and reporting elements are maintained in a living, natural environment and therefore do not suffer from lifetime disadvantages typical of most biosensing platforms. Detection of the chem/bio agent reporter (GFP) can be detected only at a specific wavelength.