Publications

Portable centrifugal microfluidic platform for nucleic acid detection

Phaneuf, C.R.; Light, Yooli K.; Tran, Huu T.; Singh, Anup K.; Koh, Chung-Yan K.

The threats of disease outbreaks and bioterrorism demand field-deployable technology capable of rapid, sensitive, and accurate diagnosis. In order to address such public health concerns, we present a portable centrifugal microfluidic platform and demonstrate sensitive detection of E. coli down to single digit starting copies using isothermal amplification via loop-mediated isothermal amplification (LAMP). The platform, which is composed of a compact optical system for laser induced fluorescence (LIF) detection, a quiet brushless motor, and an efficient non-contact heater, offers an easy-to-use system capable of performing sensitive pathogen screening in a lab-free environment.