Publications

On-chip preconcentration of proteins for picomolar detection in oral fluids

Hatch, A.V.; Herr, A.E.; Throckmorton, Daniel J.; Brennan, J.P.; Giannobile, W.V.; Singh, Anup K.

We report an automated on-chip clinical diagnostic that integrates analyte mixing, preconcentration, and subsequent detection using native polyacrylamide gel electrophoresis (PAGE) immunoanalysis. Sample proteins are concentrated > 100-fold with an in situ polymerized size exclusion membrane. The membrane also facilitates rapid mixing of reagents and sample prior to analysis. The integrated system was used to rapidly (minutes) detect immune-response markers in saliva acquired from periodontal diseased patients. Copyright © 2005 by the Transducer Research Foundation, Inc.