Publications

Modular Automated Processing System (MAPS) for analysis of biological samples

Gil, Geun-Cheol G.; Throckmorton, Daniel J.; Brennan, James S.; Schoeniger, Joseph S.; VanderNoot, Victoria A.; Fruetel, Julia A.; Branda, Steven B.

We have developed a novel modular automated processing system (MAPS) that enables reliable, high-throughput analysis as well as sample-customized processing. This system is comprised of a set of independent modules that carry out individual sample processing functions: cell lysis, protein concentration (based on hydrophobic, ion-exchange and affinity interactions), interferent depletion, buffer exchange, and enzymatic digestion of proteins of interest. Taking advantage of its unique capacity for enclosed processing of intact bioparticulates (viruses, spores) and complex serum samples, we have used MAPS for analysis of BSL1 and BSL2 samples to identify specific protein markers through integration with the portable microChemLab{trademark} and MALDI.