Publications
Microscale isoelectric fractionation using immobilized pH-specific membranes for multi-dimensional analysis
We report on advancements of our microscale isoelectric fractionation ({mu}IEFr) methodology for fast on-chip separation and concentration of proteins based on their isoelectric points (pI). We establish that proteins can be fractionated depending on posttranslational modifications into different pH specific bins, from where they can be efficiently transferred to downstream membranes for additional processing and analysis. This technology can enable on-chip multidimensional glycoproteomics analysis, as a new approach to expedite biomarker identification and verification.