Publications

Investigation of cell trajectories in chaotic flow fields generated by a helical static micromixer for rapid crosslinking kinetics

Priye, Aashish P.; Renzi, Ronald F.; Meagher, Robert M.; Wu, Meiye; Poorey, Kunal N.

Transcription factor (TF) binding pattern in chromatin provide precise and comprehensive information about the cell state. However, current analysis methods such as chromatin immunoprecipitation (ChIP) does not readily facilitate in-vivo characterization required to study short lived chromatin complexes. Here we develop a microfluidic stop flow system which is capable of mixing bacterial cells with formaldehyde on a sub-second mixing residence time scale enabling us to probe the kinetics of TF bound chromatin intermediates.