Publications

Distributed Sensor Particles for Remote Fluorescence Detection of Trace Analytes: UXO/CW

Singh, Anup K.; Schmitt, Randal L.; Johnson, Mark S.; Hargis, Philip J.; Simonson, Robert J.; Simonson, Robert J.; Schoeniger, Joseph S.; Ashley, Carol S.; Brinker, C.J.; Hance, Bradley G.

This report summarizes the development of sensor particles for remote detection of trace chemical analytes over broad areas, e.g residual trinitrotoluene from buried landmines or other unexploded ordnance (UXO). We also describe the potential of the sensor particle approach for the detection of chemical warfare (CW) agents. The primary goal of this work has been the development of sensor particles that incorporate sample preconcentration, analyte molecular recognition, chemical signal amplification, and fluorescence signal transduction within a ''grain of sand''. Two approaches for particle-based chemical-to-fluorescence signal transduction are described: (1) enzyme-amplified immunoassays using biocompatible inorganic encapsulants, and (2) oxidative quenching of a unique fluorescent polymer by TNT.