Publications

Active capture and transport of virus particles using a biomolecular motor-driven, nanoscale antibody sandwich assay

Bachand, George B.; Rivera, Susan B.; Carroll-Portillo, Amanda; Hess, Henry; Bachand, George B.

The technique of active capture and transport of virus particles using a biomolecular motor-driven, nanoscale antibody sandwich assay was described. Nanofluidic transport of macromolecules within living cells is achieved using a complex, three-dimensional network of cytoskeletal filaments and motor proteins. It is observed that glutaraldehyde crosslinking successfully linked fluorescent antibodies to MT shuttles. The application of kinesin and Ab-MT as mechanical actuators enables the development of nanofluidic systems that rely only on chemical energy for capturing and separating of target analytes from a complex solution.