Publications

A versatile and robust droplet-based microfluidic automation system for high-throughput optimization of biosynthetic pathways

Iwai, Kosuke; Wehrs, Maren; Kim, Peter W.; Sustarich, Jess; Northen, Trent R.; Martin, Hector G.; Adams, Paul D.; Singh, Anup K.

We present a novel fully-automated droplet-based microfluidic system to enable programmable combinatorial mixing, electroporation for CRISPR-based gene editing, and high-throughput screening on chip. It is highly robust and compatible with conventional liquid handler systems to interface, enabling 100 different reactions at a time with dramatically lower reagents consumption. Utilizing proposed system, we perform accelerated optimization of biosynthetic pathway of indigoidine in Escherichia coli (E. coli).