Oscar Negrete

Principal Member of the Technical Staff

(925) 294-6359

Sandia National Laboratories, California
P.O. Box 969
Livermore, CA 94551-0969

Biography

Oscar Negrete is a virologist with a special interest in emerging viruses and a focus on molecular viral-host interactions. Current model viruses of interest include Nipah virus, Ebola virus, Rift Valley fever virus, arenaviruses, flaviviruses, and alphaviruses. His research uses a broad spectrum of cellular, molecular biology, functional genomics, and nanoscience techniques to dissect host pathways involved in virus replication and deliver anti-viral cargos using nanoparticles. Pseudotyped systems are used for studying highly pathogenic viruses under BSL-2 containment. As a visiting scientist at the Lawrence Livermore National Laboratory, he has access to animal models and BSL-3 select agent research facilities. The ultimate goal of his research is to assist in the formulation of new countermeasures and diagnostics for emerging infectious disease pathogens.

Education

Bachelor’s Degree: Microbiology and Molecular Genetics, University of California-Los Angeles (1999)

Doctoral Degree: Microbiology, Immunology, and Molecular Genetics, University of California-Los Angeles (2007); Advisor: Dr. Benhur Lee

Postdoctoral Fellowships: Virology, Sandia National Laboratories (2013)

Publications

Oscar Negrete, Stefan Domino, Clifford Ho, (2021). Viral Fate and Transport for COVID-19 – NVBL https://doi.org/10.2172/1822319 Publication ID: 75925

Richard Mosesso, Colleen Courtney, Nicole Collette, Oscar Negrete, Joseph Schoeniger, (2021). Controlling CRISPR via RNA Delivery using Lipid Nanoparticles https://doi.org/10.2172/1866909 Publication ID: 78351

Brooke Harmon, Oscar Negrete, (2021). COVID-19 Vaccine Semnar https://www.osti.gov/servlets/purl/1847626 Publication ID: 77216

Jeffrey Koplow, Kent Smith, Natalia Jouravel, George Buffleben, Anupama Sinha, Oscar Negrete, Todd Barnett, R. Karnesky, (2020). Supercritical CO2 sterilization of N95 Masks https://doi.org/10.2172/1821584 Publication ID: 71222

Edwin Saada, Joseph Schoeniger, Oscar Negrete, (2019). RNA targeting CRISPR systems for transcriptional gene regulation and anti-viral countermeasure development https://www.osti.gov/servlets/purl/1643300 Publication ID: 66355

Annette LaBauve, Torri Rinker, Achraf Noureddine, Darryl Sasaki, Amy Rasley, Jeff Brinker, Oscar Negrete, (2019). Lipid-Coated Mesoporous Silica Nanoparticle Technology for Antiviral Delivery to Inhibit Encephalitic Alphavirus Infection https://www.osti.gov/servlets/purl/1640950 Publication ID: 69387

Colleen Courtney, Robert Johnston, Annette LaBauve, Nicole Collete, Saada Edwin, Oscar Negrete, Steven Branda, Kimberly Butler, Joseph Schoeniger, (2019). Nanoparticle Delivery Platforms for Controlled Inhibition of CRISPR Gene Editing https://www.osti.gov/servlets/purl/1648796 Publication ID: 68578

Annette LaBauve, Edwin Saada, Jeff Brinker, Joseph Schoeniger, Oscar Negrete, (2019). DARPA.PREPARE.poster https://www.osti.gov/servlets/purl/1639626 Publication ID: 67855

Joseph Schoeniger, Oscar Negrete, (2019). DARPA.PREPARE.slides https://www.osti.gov/servlets/purl/1639646 Publication ID: 67877

Annette LaBauve, Darryl Sasaki, C. Brinker, Oscar Negrete, Torri Rinker, Achraf Noureddine, Amy Rasley, (2018). Lipid-Coated Mesoporous Silica Nanoparticle Technology for Antiviral Delivery to Inhibit Encephalitic Alphavirus Infection https://www.osti.gov/servlets/purl/1594317 Publication ID: 59392

Oscar Negrete, Edwin Saada, Steven Strutt, C. Brinker, Joseph Schoeniger, (2018). Development and Delivery of Safe CRISPR-based Anti-Viral Countermeasures Targeting Host and Viral Genomes https://www.osti.gov/servlets/purl/1806799 Publication ID: 63396

Torri Rinker, Annette LaBauve, Rita Serda, Michael Sherman, Amy Rasley, C. Brinker, Darryl Sasaki, Oscar Negrete, (2018). Lipid-Coated Mesoporous Silica Nanoparticles for Antiviral Drug Delivery to Inhibit Encephalitic Alphavirus Infection https://www.osti.gov/servlets/purl/1510174 Publication ID: 61541

Oscar Negrete, Edwin Saada, C. Brinker, Joseph Schoeniger, (2018). DARPA annual meeting poster https://www.osti.gov/servlets/purl/1510637 Publication ID: 61850

Steven Strutt, Rachel Torrez, Emine Kaya, Oscar Negrete, Jennifer Doudna, (2018). RNA-dependent RNA targeting by CRISPR-Cas9 eLife https://doi.org/10.7554/eLife.32724 Publication ID: 59099

Robert Meagher, Aashish Priye, Yooli Light, Oscar Negrete, Cameron Ball, Sara Bird, (2017). Smart Phone Diagnostic Platform For Detection of Emerging Viral Pathogens https://www.osti.gov/servlets/purl/1488912 Publication ID: 54747

Brooke Harmon, Edwin Saada, Oscar Negrete, (2017). Identification of Broad Spectrum Inhibitors of Alphaviruses Using High-Throughput Screening https://www.osti.gov/servlets/purl/1484568 Publication ID: 54407

Torri Rinker, Annette LaBauve, Achraf Noureddine, Amy Rasley, C. Brinker, Darryl Sasaki, Oscar Negrete, (2017). Lipid-Coated Mesoporous Silica Nanoparticle Technology for the Delivery of Antivirals to Inhibit Encephalitic Alphavirus Infection https://www.osti.gov/servlets/purl/1511549 Publication ID: 54206

Aashish Priye, Sara Bird, Yooli Light, Cameron Ball, Oscar Negrete, Robert Meagher, (2017). A smartphone-based diagnostic platform for rapid detection of Zika chikungunya and dengue viruses https://doi.org/10.1038/srep44778 Publication ID: 53465

Oscar Negrete, (2017). PNNL seminar https://www.osti.gov/servlets/purl/1507944 Publication ID: 56679

Oscar Negrete, (2017). CRISPR NIRF slides https://www.osti.gov/servlets/purl/1457990 Publication ID: 56314

Kimberly Butler, Kimberly Butler, Rita Serda, Rita Serda, Achraf Noureddine, Achraf Noureddine, Ayse Muniz, Ayse Muniz, Darryl Sasaki, Darryl Sasaki, Oscar Negrete, Oscar Negrete, C. Brinker, C. Brinker, (2017). Responsive Programmable Assembly of Active Colloids for Functional Materials https://www.osti.gov/servlets/purl/1456744 Publication ID: 56073

Aashish Priye, Sara Bird, Yooli Light, Cameron Ball, Oscar Negrete, Robert Meagher, (2017). A smartphone-based diagnostic platform for rapid detection of Zika, chikungunya, and dengue viruses Scientific Reports https://doi.org/10.1038/srep44778 Publication ID: 47952

Robert Meagher, Cameron Ball, Aashish Priye, Ronald Renzi, Yooli Light, Oscar Negrete, Sara Bird, Jaideep Ray, Lark Coffey, Cody Steiner, Sara Wheeler, (2016). An Integrated Microfluidic System Enabling Real-Time Autonomous Field Surveillance for Vector-Borne Pathogens https://www.osti.gov/servlets/purl/1410769 Publication ID: 47953

Joseph Schoeniger, Oscar Negrete, Brooke Harmon, Joanne Volponi, Kevin Turner, Peter Anderson, (2016). Discovery of Protease Inhibitors for New World Alphaviruses: Building in Broad Spectrum Activity Across Sequence Variants and Low Host Off-Target Binding https://www.osti.gov/servlets/purl/1505459 Publication ID: 52063

Darryl Sasaki, Carlee Ashley, Steven Branda, C. Brinker, Eric Carnes, Adrienne Greene, Bernadette Hernandez-Sanchez, Oscar Negrete, Rekha Rao, Dorina Sava Gallis, Christina Ting, (2016). NanoCRISPR Grand Challenge LDRD ? Targeted Delivery of Medical Countermeasures https://www.osti.gov/servlets/purl/1529805 Publication ID: 51625

Christopher Grant, Jessica Techel, Sara Bird, Oscar Negrete, (2016). Validation of Novel and Known Host Factors in CRISPR Genome-wide Screening for Zika Virus https://www.osti.gov/servlets/purl/1372764 Publication ID: 51200

Jessica Techel, Christopher Grant, Sara Bird, Oscar Negrete, (2016). Validation of Novel and Known Host Factors in CRISPR Genome-wide Screening for Zika Virus https://www.osti.gov/servlets/purl/1373052 Publication ID: 51243

Robert Meagher, Oscar Negrete, Koen Van Rompay, (2016). Engineering Paper-Based Sensors for Zika Virus Trends in Molecular Medicine https://doi.org/10.1016/j.molmed.2016.05.009 Publication ID: 49980

Sierra Kaszubinski, Edwin Saada, Oscar Negrete, Brooke Harmon, (2016). Optimizing Viral Detection: Creating Fluorescent Chikungunya Virus Infectious Clones https://www.osti.gov/servlets/purl/1373158 Publication ID: 51315

Oscar Negrete, (2016). Revolutionary Platform for Rapidly Countering Emerging Biological Threats https://www.osti.gov/servlets/purl/1806529 Publication ID: 50136

Oscar Negrete, (2016). NCASM presentation https://www.osti.gov/servlets/purl/1503965 Publication ID: 50137

Sara Bird, Edwin Saada, Joseph Schoeniger, Oscar Negrete, (2016). Genome-wide CRISPR screens to identify host factors important for Zika Virus infection https://www.osti.gov/servlets/purl/1365311 Publication ID: 49669

Edwin Saada, Oscar Negrete, Brooke Harmon, (2016). Identification of Small-Molecule Inhibitors of Chikungunya Virus Using High-Throughput Screening https://www.osti.gov/servlets/purl/1365338 Publication ID: 49697

Robert Meagher, Chung Koh, Yooli Light, Huu Tran, Cameron Ball, Anup Singh, Brooke Harmon, Oscar Negrete, Sarah Wheeler, William Reisen, Lark Coffey, (2016). Rapid Closed-tube Multiplexed Detection of Viral and Bacterial Pathogens by Isothermal Amplification with Streamlined Sample Prep https://www.osti.gov/servlets/purl/1346116 Publication ID: 48666

Brooke Harmon, Anson Hatch, Oscar Negrete, (2015). Genome-Wide RNA Interference Screen Identifies Novel Drug Targets for Rift Valley Fever Virus That Minimize the Potential for Drug Resistance https://www.osti.gov/servlets/purl/1531039 Publication ID: 43531

Joseph Schoeniger, Oscar Negrete, Joanne Volponi, (2014). Designing Broad-Spectrum Antivirals https://www.osti.gov/servlets/purl/1315036 Publication ID: 37332

Carl Hayden, Oscar Negrete, Ryan Davis, (2013). Towards understanding of Nipah virus attachment protein assembly and the role of protein affinity and crowding for membrane curvature events https://doi.org/10.2172/1096477 Publication ID: 36104

Oscar Negrete, Brooke Harmon, Benjamin Schudel, Anup Singh, (2013). Poster for Sandia Research and Technology Showcase https://www.osti.gov/servlets/purl/1299212 Publication ID: 35146

Brooke Harmon, Oscar Negrete, Vinay Abhyankar, Anup Singh, (2013). Microfluidic devices for genetic screening of positionally controlled cell cultures https://www.osti.gov/servlets/purl/1078787 Publication ID: 33071

Benjamin Schudel, Brooke Harmon, Vinay Abhyankar, Benjamin Pruitt, Oscar Negrete, Anup Singh, (2013). Microfluidic platforms for RNA interference screening of virus-host interactions Lab on a Chip https://www.osti.gov/biblio/1072679 Publication ID: 30872

Oscar Negrete, Brooke Harmon, Vinay Abhyankar, Anup Singh, (2012). Microfluidic chips for RNA interference screening https://www.osti.gov/servlets/purl/1294264 Publication ID: 30227

Oscar Negrete, Carol Kozina, Mark Tucker, Jasper Hardesty, (2012). A C. elegans-based foam for rapid on-site detection of residual live virus https://doi.org/10.2172/1035339 Publication ID: 26185

Benjamin Schudel, Oscar Negrete, Brooke Harmon, Benjamin Pruitt, Anup Singh, (2011). Microfluidic devices to elucidate human gene participation in infection of rift valley fever virus 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 https://www.osti.gov/servlets/purl/1106010 Publication ID: 23662

Anup Singh, Oscar Negrete, Brooke Harmon, (2011). Microfluidic Chips for RNA Interference https://www.osti.gov/servlets/purl/1107017 Publication ID: 25284

Catherine Branda, Conrad James, Carol Kozina, Ronald Manginell, Milind Misra, Matthew Moorman, Oscar Negrete, James Ricken, Meiye Wu, (2011). Investigation of type-I interferon dysregulation by arenaviruses : a multidisciplinary approach https://doi.org/10.2172/1029825 Publication ID: 24545

Carlee Ashley, Oscar Negrete, Gregory Sommer, Steven Branda, (2011). Engineered Virus-Like Particles for Pathogen Detection Vaccination and Targeted Delivery of Therapeutics https://www.osti.gov/servlets/purl/1662129 Publication ID: 24171

Oscar Negrete, Brooke Harmon, Anup Singh, (2011). Microfluidic RNA Interference Screening Platform for Host Factors Required for Rift Valley Fever Virus Infection https://www.osti.gov/servlets/purl/1107760 Publication ID: 22958

Benjamin Schudel, Oscar Negrete, Brooke Harmon, Anup Singh, (2011). Microfluidic chips for RNA interference screening https://www.osti.gov/servlets/purl/1288922 Publication ID: 21982

Oscar Negrete, (2010). DHS intern recruitment project description https://www.osti.gov/servlets/purl/1675111 Publication ID: 20800

Matthew Moorman, James Ricken, Ronald Renzi, Ronald Manginell, Catherine Branda, Milind Misra, Oscar Negrete, Bryan Carson, (2010). A microfluidic single-cell isolation device for ensemble measurements of viral hemorrhagic fever pathogenesis in macrophages https://www.osti.gov/servlets/purl/1121687 Publication ID: 20413

Oscar Negrete, Carl Hayden, (2010). A bio-synthetic interface for discovery of viral entry mechanisms https://doi.org/10.2172/1008149 Publication ID: 19939

Catherine Branda, Carol Kozina, Oscar Negrete, Jasper Hardesty, Mark Tucker, (2010). A C. elegans-Based Foam for Rapid On-Site Detection of Residual Live Virus https://www.osti.gov/servlets/purl/1675260 Publication ID: 19698

Oscar Negrete, Brooke Harmon, Benjamin Schudel, Anup Singh, (2010). Genome-Wide RNA Interference Analysis of Viral Encephalitis Pathogenesis https://www.osti.gov/servlets/purl/1675330 Publication ID: 19390

Showing Results. Show More Publications

Research Interests

Nipah and Hendra Virus

As a graduate student at UCLA, Negrete studied the entry mechanisms of Nipah virus (NiV) and Hendra virus (HeV), two emerging zoonotic paramyoxviruses in the genus Henipavirus. NiV infection in humans is highly fatal and results primarily in severe encephalitis. In efforts to understand henipavirus pathology and zoonotic transmission, he sought to identify the host receptors. Using immunoadhesin pulldown assays, he first determined that ephrinB2, a ligand for the Eph receptor tyrosine kinases, is a functional receptor for NiV. Expression of ephrinB2 in non-permissive cells allowed for NiV fusion and entry, while soluble ephrinB2 inhibited NiV fusion and entry. EphrinB2 is an evolutionary conserved protein expressed in neurons, endothelial and smooth muscle cells, and its discovery as a receptor provided an explanation for NiV tropism and zoonosis. He then identified ephrinB3, a homolog of ephrinB2, as an alterative receptor for NiV. EphrinB3 bound to NiV-G with subnanomolar affinity, albeit at a lower efficiency than ephrinB2. EphrinB3 was sufficient to allow entry of live NiV. Furthermore, mutational analysis indicated two overlapping ephrinB2 and B3 residues as critical determinants of NiV binding and entry. Global expression analysis showed distinct ephrinB3 expression in the brain stem. Thus, ephrinB3-mediated viral entry and pathology may underlie the severe brain stem neuronal dysfunction seen in fatal Nipah encephalitis. Lastly, he compared the efficiencies of ephrin receptor usage between henipaviruses and found they had comparable ephrinB2 receptor usage. However, ephrinB3 usage by HeV was significantly reduced compared to NiV. One residue, in an ephrinB3-specific binding domain, was found to cause this reduction. The differential usage of ephrinB3 by variant HeV strains may contribute to the differential pathological profiles observed between henipaviruses. Without vaccines or effective therapeutics, henipaviruses continue to present a critical threat to global health and economies. The identification of the receptors and the development of systems used in their discovery have not only shed light on certain aspects of human pathology caused by these viruses, but have also provided new strategies for therapeutic interventions.

Relevant Publications:

Negrete OA, MC Wolf, HC Aguilar, S Enterlein, W Wang, E Muhlberger, SV Su, A Bertolotti-Ciarlet, R Flick, B Lee. (2006). Two key residues in ephrinB3 are critical for its use as an alternative receptor for Nipah virus. PLoS Pathogens 2:e7.
Negrete OA, D Chu, HC Aguilar, B Lee. (2007). Single amino acid changes in the Nipah and Hendra virus attachment glycoprotein distinguishes ephrinB2 from ephrinB3 usage. Journal of Virology 81:10804.
Lee, B, OA Negrete. Henipavirus receptor and uses thereof. International Patent publication WO/2007/005244. Jan 11, 2007.
Maar D, Harmon B, Chu D, Shultz B, Aguilar HC, Lee B, Negrete OA*. (2012) Cysteines in the stalk domain of the Nipah virus G glycoprotein are located in a distinct subdomain critical for fusion activation. Journal of Virology 86(12):6632-42. *Corresponding author

Virology and Rift Valley Fever Virus

As a postdoctoral appointee, Negrete was hired at Sandia National Laboraotories to establish virology research in a department whose interests were mainly engineering, microfluidics, and diagnostics. Early in his postdoc, Negrete was awarded an internal grant to conduct virology research as a principal investigator and led a team of postdocs and technicans on a project focused on functional genomics screening using RNA interference (RNAi). Genome-wide RNAi screening offers a systematic investigation of critical cellular hostpathogen interactions, which in turn can serve to inform host-direct countermeasure development. Using this method against Rift Valley fever virus (RVFV), a biodefense threat listed as a category A priority pathogen, he identified initial candidate genes that helped dissect RVFV entry. To determine the cellular entry mechanism of RVFV, he used small-molecule inhibitors and dominant negative protein expression, in addition to RNAi to inhibit the major mammalian cell endocytic pathways. He found that the primary mechanism of RVFV MP-12 uptake is dynamin-dependent, caveolin-1-mediated endocytosis. These results were in contrast to the original assumptions in the field prior to this study that suggested RVFV entry was mediated by clathrin dependent endocytosis.

By instituting the procedures for RNAi screening using traditional methodology, Negrete then integrated with the engineering team at Sandia to develop a miniaturized microfludic RNAi screening platform. Negrete and his team developed a two part microfluidic system for patterning and screening gene targets on-chip to examine cellular pathways involved in virus entry using polydimethylsiloxane (PDMS)-based platforms. Validation of the screening platform was examined using Vesicular stomatitis virus and RVFV. The ultimate vision for this project was to enable large scale screening in high containment biosafety laboratories with portable inexpensive microfluidic platforms.

Lastly, using bioinformatic analysis of our entire genome wide screening results against RVFV, they found that the Wnt pathway was the most represented pathway when gene hits were functionally clustered. With further investigation, they found that RVFV infection activated Wnt signaling, was enhanced when Wnt signaling was pre-activated, was reduced with knockdown of β-catenin, and was blocked using Wnt signaling inhibitors. Similar results were found using distantly related bunyaviruses La Crosse virus and California encephalitis virus, suggesting a conserved role for Wnt signaling in bunyaviral infection. They ended up proposing a model where bunyaviruses activated Wnt responsive genes to regulate optimal cell cycle conditions needed to promote efficient viral replication.

Relevant Publications:

Schudel BR, Harmon B, Abhyankar V, Pruitt B, Negrete OA*, Singh AK*. (2013) Microfluidic platforms for RNA Interference screening of virus-host interactions. Lab on a chip. Feb 5;13(5):811-7 (Back cover) *Co-Corresponding author.
Perroud T, Renzi R, Negrete O, Claudnic MR. A Microelectroporation device for genomic screening.U.S. Patent 8,828,736. September 9,2014.
Harmon B, Bird SW, Schudel BR, Hatch AV, Rasley A, Negrete OA* (2016) A genome-wide RNA interference screen identifies a role for Wnt/beta-catenin signaling during Rift Valley fever virus infection. Journal of Virology. July27;90(16):7084-97. *Corresponding author

Viral Research Models

As a permanent staff member at Sandia National Labs, Negrete has expanded Sandia’s catalog of viral research models and established collaborations for high containment-select agent research and for developing novel diagnostics, countermeasures and in vivo delivery vehicles to detect and treat biodefense and emerging viral diseases. Negrete and his team have now published several peer review manuscripts using various viral model systems that include henipaviruses, buynaviruses, arenaviruses, flaviviruses, alphaviruses, and filoviruses. Through a DTRA funded effort, they established collaborations with Dr. Amy Rasley at the Lawrence Livermore National Laboratory (LLNL) and with Dr. Jeff Brinker, a Distinguished and Regent’s Professor at the University of New Mexico (UNM) who has made numerous pioneering contributions to processing, characterization, and understanding of porous and composite nanostructured materials. The DTRA project goals were to develop lipid-coated mesoporous silica nanoparticles (LC-MSNs) as antiviral delivery vehicles targeting the central nervous system aimed at inhibiting encephalitic alphavirus infections. The large surface area of the MSN core promotes hydrophobic drug loading while the liposome coating retains the drug and enables enhanced circulation time and biocompatibility, providing an ideal platform for antiviral drug delivery. At LLNL, in vitro antiviral testing was performed at BSL-3 against Venezuelan equine encephalitis virus (VEEV) and in vivo testing was performed using a vaccine strain of VEEV under ABSL-2 containment. Antiviral-loaded LC-MSNs showed significant reduction of brain viral titer in VEEV TC-83 infected mice as compared to PBS treated controls and the manuscript describing the results is currently under review.

Negrete has also recently established collaboration with Jennifer Doudna, a professor at the University of California, Berkeley (UCB), HHMI investigator, Director of the Innovative Genomics Institute, and co-inventor of CRISPR-Cas9 genome-editing technology. Together, they have a DARPA-sponsored effort to develop CRISPRbased countermeasures to inhibit viral infection of biodefense concern, develop anti-CRISPR proteins to regulate genome editing activity, and to refine LC-MSN technology for in vivo CRISPR and anti-CRISPR cargo delivery. They have recently published an article in eLife describing RNA-dependent RNA targeting by CRISPRCas9 enzymes for Staphylococcus aureus and Campylobacter jejuni. Using the rules defined in this study, they have enabled transcriptional control in mammalian cells using RNA targeting Cas9 systems.

Relevant Publication:

Harmon B, Kozina C, Maar D, Carpenter T, Branda C, Carson B#, Negrete OA*#. (2013) Identification of amino acids within the nucleoprotein of Tacaribe virus important for anti interferon activity. Journal of Biological Chemistry. March22;288(12):8702-11 *Corresponding author, # Contributed equally
Ashely C, Brinker CJ, Carnes EC, Fekrazad MH, Felton LA, Negrete O, Padilla DP, Wilkinson BS, Wilkinson DC, Willman CL. Porous nanoparticle-supported lipid bilayers (protocells) for targeted delivery including transdermal delivery of cargo and methods thereof. International Patent publication WO 2013056132. April 18, 2013.
Meagher RJ, Negrete OA, Van Rompay KK. (2016) Engineering paper-based sensors for Zika virus.Trends in Molecular Medicine. July;22(7):529-30
Malmlov A, Seetahal J, Carrington C, Ramkisson V, Foster J, Miazgowicz KL, Quackenbush S, Rovnak J, Negrete OA, Munster V, Schountz T. (2017) Serological evidence of arenavirus circulation among fruit bats in Trinidad. PLoS ONE 12(9). e0185308
LaBauve A, Rinker T, Noureddine A, Serda R, Sherman M, Rasley A, Brinker CJ, Negrete OA. (2018) Lipid-coated mesoporous silica nanoparticles for the delivery of the ML336 antiviral to inhibit encephalitic alphavirus infection. Scientific Reports.

Relevant Prior Positions

Graduate Student Researcher, University of California Los Angeles (2003-2007)
Postdoctoral Appointee, Sandia National Laboratories (2008-2013)
Senior Member of the Technical Staff, Sandia National Laboratories (2013-2018)
Visiting Scientist, Livermore National Laboratory (2013-Present)
Principal Member of Technical Staff, Sandia National Laboratories (2018-Present)

Honors and Memberships

NIH Training Grant in Cellular Molecular Biology (2003-2006)
UCLA Dissertation Year Fellowship (2006-2007) NIH Training Grant in Virology and Gene Therapy (2007-2008)
Gordon Conference Carl Storm Underrepresented Minority Fellowship (2009)
Sandia Post-Doctoral Technical Showcase Award (2010) Sandia Employee Recognition Award (2013)
Hispanic Engineer National Achievement Award Conference Nomination (2015, 2016)
FLC Outstanding Technology Development Award – SmartLamp Team (2017)
Full Member, American Society for Microbiology (2013-Present)
Full Member, American Society for Virology (2013-Present)