Steven Branda

Principal Member of Technical Staff

(925) 294-6751

Sandia National Laboratories, New Mexico
P.O. Box 5800
Albuquerque, NM 87185-9292

Biography

Steven Branda is a microbiologist and molecular geneticist with extensive experience in the characterization of bacterial pathogens and their interactions with the host. He has led and contributed to a wide variety of productive research projects, many of which have focused on development and application of specialized sample preparation for time- and cost-efficient analysis of host and pathogen transcriptomes via Next Generation Sequencing (NGS). Other projects have focused on development of microfluidics-based technologies for automated and integrated analysis of complex samples, including clinical specimens, for applications in detection and diagnosis of infectious diseases. His current focus is development of host-directed, CRISPR-based therapies for infectious disease. In collaboration with Dr. Sahar El-Etr, Dr. Magdalena Franco, and Dr. Nicole Collette at Lawrence Livermore National Laboratory (LLNL), he has established and characterized in vitro and in vivo models of Burkholderia thailandensis infection, and in vitro models of B. pseudomallei infection.

Education

Bachelor’s Degree: Psychobiology, Swarthmore College (1991)

Doctoral Degree: Genetics, Yale University (1999)

Postdoctoral Fellowships:

Molecular Biology, Yale University (1999-2000)
Microbiology, Harvard Medical School (2000-2005)

As a postdoctoral fellow in the laboratory of Dr. Roberto Kolter (Harvard Medical School), and in collaboration with postdoctoral fellows in the laboratory of Dr. Richard Losick (Harvard University), Branda established and extensively characterized several complementary models of biofilm formation by the Gram-positive bacterium B. subtilis. Prior to this effort, virtually all studies of bacterial biofilms focused on those produced by Gram-negative bacteria. By describing the defining features of B. subtilis biofilms in detail and, through a combination of genetics and biochemistry, elucidating the major molecular mechanisms and regulators driving their formation, Branda and his colleagues seeded a new branch of biofilm research that continues to flourish to this day.

Research Interests

NGS-enabled Analysis of Pathogen and Host Transcriptomes

The advent of NGS has made possible quantitative analysis of virtually all transcripts produced by a cell, tissue, or organism. However, brute-force NGS of complex samples is often not time or cost-efficient, particularly when the transcripts of interest are rare (e.g., produced by a pathogen that has infected host cells). In collaboration with colleagues at Sandia National Laboratories, Branda has developed novel sample preparation methods that enable suppression of highly abundant nucleic acids (e.g., molecular normalization) and enrichment of rare nucleic acids (e.g., probe-mediated capture of pathogen transcripts) for selective NGS analysis. Branda and his colleagues have used these methods for transcriptome profiling of B. pseudomallei and B. thailandensis during infection of human primary cells (manuscript in preparation), of Francisella tularensis and Yersinia enterocolitica during infection of mouse cell lines, and of B. thailandensis during respiratory infection of mice (manuscript in preparation). Additionally, they have used these methods for highly-sensitive detection of viral and bacterial pathogens in blood samples (manuscript in preparation). Others in the field have used their methods for a wide variety of applications.

Relevant Publications:

VanderNoot VA, Langevin SA, Solberg OD, Lane PD, Curtis DJ, Bent ZW, Williams KP, Patel KD, Schoeniger JS, Branda SS, Lane TW (2012) cDNA normalization by hydroxyapatite chromatography to enrich transcriptome diversity in RNA-Seq applications. BioTechniques 53:373-80.
Bent ZW, Tran-Gyamfi MB, Langevin SA, Brazel DM, Hamblin RY, Branda SS, Patel KD, Lane TW, Vandernoot VA (2013) Enriching pathogen transcripts from infected samples: A capture-based approach to enhanced host-pathogen RNA sequencing. Anal Biochem 438:90-6.
Bent ZW, Brazel DM, Tran-Gyamfi MB, Hamblin RY, VanderNoot VA, Branda SS (2013) Use of a capture-based pathogen transcript enrichment strategy for RNA-Seq analysis of the Francisella tularensis LVS transcriptome during infection of murine macrophages. PLoS ONE 8:e77834.
Bent ZW, Poorey K, Brazel DM, LaBauve AE, Sinha A, Curtis DJ, House SE, Tew KE, Hamblin RY, Williams KP, Branda SS, Young GM, Meagher RJ (2015) Transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of extracellular and intracellular survival. Infect Immun 83:2672-85

Microfluidics-Enabled Sample Preparation for Biochemical Analyses

Reliable handling, fractionation, and modification of small biological samples can be exceedingly difficult when using conventional methods. Microfluidics-based methods offer improved precision, and often can be fully automated, but integrating multiple manipulations that together constitute a sample processing pipeline can be challenging, and usually requires a complicated platform architecture (e.g., robotics). In collaboration with colleagues at Sandia National Laboratories, Branda has developed and validated a variety of microfluidics-based devices for processing of small biological samples (bacteria, mammalian cells, and clinical specimens) for NGS, flow cytometry, and mass spectrometry analyses. In many cases this required integration of continuous-flow and digital modalities of microfluidics, as well as rational redesign of conventional techniques for microscale implementation. Branda and his colleagues’ approaches to these problems have been adopted by many researchers, and many of their devices and techniques have been patented and licensed.

Relevant Publications:

Srivastava N, Brennan JS, Renzi RF, Wu M, Branda SS, Singh AK, Herr AE (2009) Fully integrated microfluidic platform enabling automated phosphoprofiling of macrophage response. Anal Chem 81:3261-9.
Wu M, Perroud TD, Srivastava N, Branda CS, Sale KL, Carson BD, Patel KD, Branda SS, Singh AK (2012) Microfluidically-unified cell culture, sample preparation, imaging, and flow cytometry for measurement of cell signaling pathways with single cell resolution. Lab Chip 12:2823-31.
Kim H, Jebrail MJ, Sinha A, Bent ZW, Solberg OD, Williams KP, Langevin SA, Renzi RF, Van de Vreugde JL, Meagher RJ, Schoeniger JS, Lane TW, Branda SS, Bartsch MS, Patel KD (2013) A microfluidic DNA library preparation platform for next-generation sequencing. PLoS ONE 8:e68988.
Jebrail MJ, Sinha A, Vellucci S, Renzi RF, Ambriz C, Gondhalekar C, Schoeniger JS, Patel KD, Branda SS (2014) World-to-digital-microfluidics interface enabling extraction and purification of RNA from human whole blood. Anal Chem 86:3856-62.

Publications

Steven Branda, Nicole Collette, Nicole Aiosa, Neha Garg, Catherine Mageeney, Kelly Williams, Ashlee Phillips, Kelsey Hern, Adam Arkin, James Ricken, Delaney Wilde, Sahiba Dogra, Brittany Humphrey, Kunal Poorey, Colleen Courtney, (2022). Reconfiguration of the Respiratory Tract Microbiome to Prevent and Treat Burkholderia Infection https://doi.org/10.2172/1898252 Publication ID: 80404

Nicole Aiosa, Anupama Sinha, Olakunle Jaiyesimi, Ricardo da Silva, Steven Branda, Neha Garg, (2022). Metabolomics Analysis of Bacterial Pathogen Burkholderia thailandensis and Mammalian Host Cells in Co-culture ACS Infectious Diseases https://doi.org/10.1021/acsinfecdis.2c00233 Publication ID: 80918

Steven Branda, Richard Mosesso, Anupama Sinha, Christine Thatcher, Nicole Collette, Ashlee Phillips, Tanya Tanner, (2021). Nanoparticle Mediated Delivery of Therapeutic mRNA for Protection Against Lung Damage https://doi.org/10.2172/1891377 Publication ID: 80332

Matthew Hirakawa, Nikki Tjahjono, Yooli Light, Prem Chintalapudi, Steven Branda, Kimberly Butler, Raga Krishnakumar, (2021). Augmentation of Antibacterial Activity in Mesenchymal Stromal Cells Through Systems-Level Analysis and CRISPR-mediated Activation of CD14 https://doi.org/10.2172/1868446 Publication ID: 78467

Nikki Aiosa, Anupama Sinha, Steven Branda, Neha Garg, (2021). Reconfiguring Respiratory Tract Microbiomes to Combat Infection https://www.osti.gov/servlets/purl/1859836 Publication ID: 77776

Danae Maes, Jerilyn Timlin, Stephen Anthony, Joshua Podlevsky, Steven Branda, (2020). Concurrent evaluation of autophagy induction and Burkholderia infection at the single cell level https://www.osti.gov/servlets/purl/1831029 Publication ID: 71479

Steven Branda, Kunal Poorey, (2020). COVID-19 biomarkers based on respiratory microbiome content https://doi.org/10.2172/1673815 Publication ID: 71018

Catherine Mageeney, Anupama Sinha, Kelly Williams, Steven Branda, (2020). Genome Sequences of Burkholderia thailandensis Strains E421, E426, and DW503 Microbiology Resource Announcements https://doi.org/10.1128/MRA.00312-20 Publication ID: 71061

Matthew Hirakawa, Nikki Tjahjono, Yooli Light, Prem Chintalapudi, Kimberly Butler, Steven Branda, Raga Krishnakumar, (2020). Engineering mesenchymal stromal cells for anti-microbial therapy https://www.osti.gov/servlets/purl/1768163 Publication ID: 72795

Colleen Courtney, Anupama Sinha, Debjit Ray, Jessica Techel, Kunal Poorey, Nicole Collette, Ashlee Phillips, Sahar El-Etr, Magdalena Franco, Steven Branda, (2019). Host-directed CRISPR based countermeasures for Burkholderia https://www.osti.gov/servlets/purl/1643291 Publication ID: 66338

Raga Krishnakumar, Catherine Branda, Steven Branda, Kimberly Butler, Nikki Tjahjono, Yooli Light, (2019). Characterizing and Engineering Mesenchymal Stromal Cells for Anti-Microbial Therapy https://www.osti.gov/servlets/purl/1643235 Publication ID: 66184

Steven Branda, Pei-Li Wang, Annette LaBauve, Anupama Sinha, Kunal Poorey, Kelly Williams, George Michailidis, Joseph Schoeniger, Catherine Mageeney, Colleen Courtney, Sahar El-Etr, Magda Franco, Victoria Lao, Patrik D’haeseleer, Jose Pena, Brent Segelke, (2019). Elucidation of Host-Pathogen Interactions via Dual RNA-Seq Analysis to Support Development of Countermeasures Against the Intracellular Bacterial Pathogen Burkholderia pseudomallei https://www.osti.gov/servlets/purl/1643632 Publication ID: 66457

Catherine Mageeney, Britney Lau, Steven Branda, Anupama Sinha, Raga Krishnakumar, Kelly Williams, (2019). Computational front end to diverse therapeutic phage cocktails https://www.osti.gov/servlets/purl/1643273 Publication ID: 66274

Robert Johnston, Kyle Seamon, Edwin Saada, Joshua Podlevsky, Steven Branda, Jerilyn Timlin, Jason Harper, (2019). Use of anti-CRISPR protein AcrIIA4 as a capture ligand for CRISPR/Cas9 detection Biosensors and Bioelectronics https://doi.org/10.1016/j.bios.2019.111361 Publication ID: 70297

Catherine Mageeney, Alecia Rokes, Britney Lau, Steven Branda, Anupama Sinha, Raga Krishnakumar, Kelly Williams, (2019). Computational front end to diverse therapeutic phage cocktails https://www.osti.gov/servlets/purl/1641506 Publication ID: 70235

Prem Chintalapudi, Matthew Hirakawa, Nikki Tjahjono, Steven Branda, Kimberly Butler, Raga Krishnakumar, (2019). CRISPR-based Genetic Engineering of Mesenchymal Stem Cells https://www.osti.gov/servlets/purl/1641290 Publication ID: 69980

Robert Johnston, Edwin Saada, Joshua Podlevsky, Steven Branda, Jason Harper, Jerilyn Timlin, (2019). Anti-CRISPR Based Platform for Rapid Detection and Quantification of Cas9-RNP https://www.osti.gov/servlets/purl/1640979 Publication ID: 69447

Catherine Mageeney, Kelly Williams, Steven Branda, Raga Krishnakumar, (2019). Diverse therapeutic phage cocktails through a computational front end https://www.osti.gov/servlets/purl/1640653 Publication ID: 68942

Catherine Mageeney, Alecia Rokes, Britney Lau, Steven Branda, Raga Krishnakumar, Kelly Williams, (2019). Computational front end to diverse therapeutic phage cocktails https://www.osti.gov/servlets/purl/1640900 Publication ID: 69317

Colleen Courtney, Robert Johnston, Annette LaBauve, Nicole Collete, Saada Edwin, Oscar Negrete, Steven Branda, Kimberly Butler, Joseph Schoeniger, (2019). Nanoparticle Delivery Platforms for Controlled Inhibition of CRISPR Gene Editing https://www.osti.gov/servlets/purl/1648796 Publication ID: 68578

Catherine Mageeney, Alecia Rokes, Briney Lau, Steven Branda, Raga Krishnakumar, Kelly Williams, (2019). Diverse therapeutic phage cocktails through a computational front end https://www.osti.gov/servlets/purl/1639435 Publication ID: 67521

Nikki Tjahjono, Matthew Hirakawa, Yooli Light, Steven Branda, Kimberly Butler, (2019). Characterizing and Engineereing Mesenchymal Stem Cells for Antimicrobial Therapy https://www.osti.gov/servlets/purl/1602946 Publication ID: 67212

Raga Krishnakumar, Anupama Sinha, Sara Bird, Harikrishnan Jayamohan, Harrison Edwards, Joseph Schoeniger, Kamlesh Patel, Steven Branda, Michael Bartsch, (2018). Systematic and stochastic influences on the performance of the MinION nanopore sequencer across a range of nucleotide bias Scientific Reports https://doi.org/10.1038/s41598-018-21484-w Publication ID: 58764

Philip Miller, Steven Branda, Ronen Polsky, Justin Baca, (2018). Extraction and biomolecular analysis of dermal interstitial fluid collected with hollow microneedles Communications Biology https://doi.org/10.1038/s42003-018-0170-z Publication ID: 80709

Michael Bartsch, Sara Bird, Steven Branda, Harrison Edwards, Harikrishnan Jayamohan, Raga Krishnakumar, Kamlesh Patel, Joseph Schoeniger, Anupama Sinha, (2018). Real-Time Automated Pathogen Identification by Enhanced Ribotyping (RAPIER) LDRD Final Report https://doi.org/10.2172/1481615 Publication ID: 59412

Steven Branda, Colleen Courtney, Anupama Sinha, Kunal Poorey, Joseph Schoeniger, Annette LaBauve, Kelly Williams, (2018). Elucidation & manipulation of host-pathogen interactions for development of countermeasures against intracellular bacterial pathogen Burkholderiapseudomallei https://www.osti.gov/servlets/purl/1594641 Publication ID: 59351

Colleen Courtney, Anupama Sinha, Kunal Poorey, Steven Branda, Debjit Ray, Nicole Collette, Ashlee Fergusen, Sahar El-Etr, (2018). Elucidating Genetic Mechanisms of Bacterial Pathogenesis for Host-Directed Protection https://www.osti.gov/servlets/purl/1594329 Publication ID: 59389

Yooli Light, Matthew Hirakawa, Nikki Tjahjono, Steven Branda, Kimberly Butler, Raga Krishnakumar, (2018). Mesenchymal stem cell-based therapy for infectious disease https://www.osti.gov/servlets/purl/1594331 Publication ID: 59391

Michael Bartsch, Steven Branda, Raga Krishnakumar, Anupama Sinha, Harrison Edwards, Sara Bird, Harikrishnan Jayamohan, Kamlesh Patel, (2018). LDRD Ending Project ReviewReal-time Automated Pathogen Identification by Enhanced Ribotyping (RAPIER) https://www.osti.gov/servlets/purl/1806834 Publication ID: 58981

Robert Johnston, Kyle Seamon, Edwin Saada, Joshua Podlevsky, Steven Branda, Jason Harper, Jerilyn Timlin, (2018). Rapid Electrochemical Detection and Quantification of CRISPR/Cas9 Components https://www.osti.gov/servlets/purl/1582212 Publication ID: 63819

Jason Harper, Robert Johnston, Kyle Seamon, Edwin Saada, Steven Branda, Jerilyn Timlin, (2018). Rapid Electrochemical Detection of CRISPR/Cas9 Components using anti-CRISPR Protein AcrIIA4 as Capture Ligand https://www.osti.gov/servlets/purl/1581727 Publication ID: 63690

Britney Lau, Catherine Mageeney, Steven Branda, Kelly Williams, (2018). Phage Therapy against Pseudomonas aeruginosa in a Galleria mellonella infection model https://www.osti.gov/servlets/purl/1806628 Publication ID: 63262

Michael Bartsch, Raga Krishnakumar, Anupama Sinha, Joseph Schoeniger, Steven Branda, Kamlesh Patel, Harrison Edwards, Sara Bird, Harikrishnan Jayamohan, (2018). Characterizing the Performance of the MinION for Real-Time Detection https://www.osti.gov/servlets/purl/1503084 Publication ID: 61161

Raga Krishnakumar, Michael Bartsch, Kamlesh Patel, Steven Branda, Joseph Schoeniger, Harrison Edwards, Sara Bird, Harikrishnan Jayamohan, (2017). Selective Long-Read Nanopore Sequencing for Real-Time Point-of-need Pathogen Identification https://www.osti.gov/servlets/purl/1511977 Publication ID: 54662

Britney Lau, Raga Krishnakumar, Julian Wagner, Anupama Sinha, Corey Hudson, Joseph Schoeniger, Steven Branda, Kelly Williams, (2017). Discovering and converting temperate phages for therapy https://www.osti.gov/servlets/purl/1483224 Publication ID: 54346

Harikrishnan Jayamohan, Michael Bartsch, Raga Krishnakumar, Kamlesh Patel, Steven Branda, Anupama Sinha, Harrison Edwards, Daniel Throckmorton, Sara Bird, Joseph Schoeniger, (2017). Nanopore Sequencing for Real-Time Pathogen Identification https://www.osti.gov/servlets/purl/1507629 Publication ID: 55103

Joseph Schoeniger, Debjit Ray, Steven Branda, Kelly Williams, Corey Hudson, Christopher Polage, (2016). Cheap and Fast Multiplexed Bacterial Genomic Sequencing: Enabling Comparative Genomic Analysis of Antibiotic Resistance in BWA and Hospital Acquired Infections https://www.osti.gov/servlets/purl/1505457 Publication ID: 52119

Darryl Sasaki, Carlee Ashley, Steven Branda, C. Brinker, Eric Carnes, Adrienne Greene, Bernadette Hernandez-Sanchez, Oscar Negrete, Rekha Rao, Dorina Sava Gallis, Christina Ting, (2016). NanoCRISPR Grand Challenge LDRD ? Targeted Delivery of Medical Countermeasures https://www.osti.gov/servlets/purl/1529805 Publication ID: 51625

Debjit Ray, Joseph Schoeniger, Kelly Williams, Rachelle Hamblin, Anupama Sinha, Corey Hudson, Steven Branda, (2016). Genome-Based Prediction pathogenic potential of the new ?super bug?: Clostridium difficile https://www.osti.gov/servlets/purl/1365235 Publication ID: 49554

Patrik D’haeseleer, Shannon Johnson, Karen Davenport, Patrick Chain, Joseph Schoeniger, Debjit Ray, Anupama Sinha, Kelly Williams, José Peña, Steven Branda, Sahar El-Etr, (2016). Genome sequence of the historical clinical isolate Burkholderia pseudomallei PHLS 6 Genome Announcements https://doi.org/10.1128/genomeA.00649-16 Publication ID: 50052

Mais Jebrail, Anupama Sinha, Ronald Renzi, James Van De Vreugde, Carmen Gondhalekar, Joseph Schoeniger, Steven Branda, (2015). Automated Preparation of Nucleic Acids from Blood for Point-of-Care Applications https://www.osti.gov/servlets/purl/1531051 Publication ID: 43434

Kunal Poorey, Anupama Sinha, Deanna Curtis, Kelly Williams, Steven Branda, Robert Meagher, (2015). A transcriptomic analysis of Yersinia enterocolitica biovar 1B infecting murine macrophages reveals new mechanisms of intracellular survival Infection and Immunity https://doi.org/10.1128/IAI.02922-14 Publication ID: 40615

Michael Bartsch, Harrison Edwards, Daniel Lee, Caroline Moseley, Karen Tew, Ronald Renzi, James Van De Vreugde, Hanyoup Kim, Daniel Knight, Anupama Sinha, Steven Branda, Kamlesh Patel, (2015). The rotary zone thermal cycler: A low-power system enabling automated rapid PCR PLoS ONE https://doi.org/10.1371/journal.pone.0118182 Publication ID: 37945

Steven Branda, (2015). BloodPrep RNA MedTech Showcase Presentation https://www.osti.gov/servlets/purl/1240338 Publication ID: 42294

Mais Jebrail, Ronald Renzi, Anupama Sinha, James Van De Vreugde, Carmen Gondhalekar, Cesar Ambriz, Robert Meagher, Steven Branda, (2015). A solvent replenishment solution for managing evaporation of biochemical reactions in air-matrix digital microfluidics devices Lab on a Chip https://doi.org/10.1039/c4lc00703d Publication ID: 41221

Steven Branda, Mais Jebrail, Anupama Sinha, Ronald Renzi, Michael Bartsch, James Van De Vreugde, Carmen Gondhalekar, Cesar Amriz, Joseph Schoeniger, Robert Meagher, Kamlesh Patel, (2015). Advanced Diagnostic and Sample Preparation Platform for Early Threat Surveillance https://doi.org/10.2172/1490544 Publication ID: 41223

Anupama Sinha, Deanna Curtis, Steven Branda, Robert Meagher, (2013). A capture-based technique for enhancing RNA-Seq analysis of bacterial transcriptomes during in vitro and in vivo infections https://www.osti.gov/servlets/purl/1120640 Publication ID: 36705

Steven Branda, (2013). Advanced Diagnostic and Sample Preparation Platform for Early Threat Surveillance 158814 https://www.osti.gov/servlets/purl/1673685 Publication ID: 36542

Mais Jebrail, Anupama Sinha, Ronald Renzi, Kamlesh Patel, Steven Branda, (2013). A World-to-Digital Microfluidic Interface for Whole Blood RNA Preparation https://www.osti.gov/servlets/purl/1115237 Publication ID: 36325

Anupama Sinha, Deanna Curtis, Steven Branda, (2013). RNA-Seq analysis of the Yersinia enterocolitica biovar 1B transcriptome at early time points during the infection of murine macrophage cells https://www.osti.gov/servlets/purl/1314273 Publication ID: 35785

Hanyoup Kim, Mais Jebrail, Anupama Sinha, Zachary Bent, Owen Solberg, Kelly Williams, Stanley Langevin, Ronald Renzi, James Van De Vreugde, Robert Meagher, Joseph Schoeniger, Todd Lane, Steven Branda, Michael Bartsch, Kamlesh Patel, (2013). A Microfluidic DNA Library Preparation Platform for Next-Generation Sequencing PLoS ONE https://www.osti.gov/biblio/1072475 Publication ID: 32780

David Brazel, Mary Tran-Gyamfi, Victoria VanderNoot, Steven Branda, (2013). An RNA-Seq analysis of Francisella tularensis LVS transcriptional shifts at early time points during in vitro infections PLoS One https://www.osti.gov/biblio/1113046 Publication ID: 34364

Zachary Bent, Mary Tran-Gyamfi, Stanley Langevin, David Brazel, Steven Branda, Todd Lane, Victoria VanderNoot, (2013). Enriching pathogen transcripts from infected samples: A capture-based approach to enhanced host–pathogen RNA sequencing Analytical Biochemistry https://doi.org/10.1016/j.ab.2013.03.008 Publication ID: 31337

Mary Tran-Gyamfi, Stanley Langevin, David Brazel, Steven Branda, Todd Lane, Victoria VanderNoot, (2013). Enriching pathogen transcripts from infected samples : https://www.osti.gov/servlets/purl/1503472 Publication ID: 32686

Zachary Bent, Mary Tran-Gyamfi, Stanley Langevin, David Brazel, Steven Branda, Todd Lane, Victoria VanderNoot, (2013). Enriching pathogen transcripts from infected samples : https://www.osti.gov/servlets/purl/1503470 Publication ID: 32332

Zachary Bent, Steven Branda, (2013). The Yersinia enterocolitica Ysa Type III Secretion System is Expressed during Infections both In Vitro and In Vivo Proposed for publication in PLoS Pathogens. https://www.osti.gov/biblio/1063601 Publication ID: 31421

Stanley Langevin, Todd Lane, Pamela Lane, Zachary Bent, Owen Solberg, Deanna Curtis, Kelly Williams, Steven Branda, Kamlesh Patel, Joseph Schoeniger, (2012). cDNA normalization by hydroxyapatite chromatography to enhance transcriptome diversity in RNA-Seq Applications Biotechniques https://doi.org/10.2144/000113937 Publication ID: 30653

Todd Lane, Kelly Williams, Owen Solberg, Kamlesh Patel, Steven Branda, Stanley Langevin, Pamela Lane, Zachary Bent, Deanna Curtis, Mary Tran-Gyamfi, Victoria VanderNoot, Joseph Schoeniger, (2012). Rapid Threat Organism Recognition https://www.osti.gov/biblio/1067717 Publication ID: 28455

Steven Branda, Mais Jebrail, James Van De Vreugde, Stanley Langevin, Zachary Bent, Deanna Curtis, Pamela Lane, Bryan Carson, Elisa La Bauve, Kamlesh Patel, James Ricken, Joseph Schoeniger, Owen Solberg, Kelly Williams, Milind Misra, Amy Powell, Nicholas Pattengale, Elebeoba May, Todd Lane, Duane Lindner, Malin Young, Victoria VanderNoot, Numrin Thaitrong, Michael Bartsch, Ronald Renzi, Mary Tran-Gyamfi, Robert Meagher, (2012). Characterization of Pathogens in Clinical Specimens via Suppression of Host Background for Efficient Second Generation Sequencing Analyses https://www.osti.gov/servlets/purl/1141142 Publication ID: 28871

Steven Branda, Mais Jebrail, James Van De Vreugde, Stanley Langevin, Zachary Bent, Deanna Curtis, Pamela Lane, Bryan Carson, Elisa La Bauve, Kamlesh Patel, James Ricken, Joseph Schoeniger, Owen Solberg, Kelly Williams, Milind Misra, Amy Powell, Nicholas Pattengale, Elebeoba May, Todd Lane, Duane Lindner, Malin Young, Victoria VanderNoot, Numrin Thaitrong, Michael Bartsch, Ronald Renzi, Mary Tran-Gyamfi, Robert Meagher, (2012). Copy of Automated Molecular Biology Platform Enabling Rapid & Efficient SGS Analysis of Pathogens in Clinical Samples https://www.osti.gov/servlets/purl/1688623 Publication ID: 27929

Steven Branda, Mais Jebrail, James Van De Vreugde, Stanley Langevin, Zachary Bent, Deanna Curtis, Pamela Lane, Bryan Carson, Elisa La Bauve, Kamlesh Patel, James Ricken, Joseph Schoeniger, Owen Solberg, Kelly Williams, Milind Misra, Amy Powell, Nicholas Pattengale, Elebeoba May, Todd Lane, Duane Lindner, Malin Young, Victoria VanderNoot, Numrin Thaitrong, Michael Bartsch, Ronald Renzi, Mary Tran-Gyamfi, Robert Meagher, (2012). Automated Molecular Biology Platform Enabling Rapid & Efficient SGS Analysis of Pathogens in Clinical Samples https://www.osti.gov/biblio/1067707 Publication ID: 27930

Stanley Langevin, Zachary Bent, Owen Solberg, Deanna Curtis, Pamela Lane, Kelly Williams, Joseph Schoeniger, Todd Lane, Steven Branda, (2012). Peregrine : https://www.osti.gov/servlets/purl/1294360 Publication ID: 27691

Stanley Langevin, Victoria VanderNoot, Owen Solberg, Deanna Curtis, Mary Tran-Gyamfi, Pamela Lane, Steven Branda, Todd Lane, (2012). Negative capture combined with HAC normalization for selective enrichment of pathogen-derived nucleic acids in clinical samples https://www.osti.gov/servlets/purl/1294283 Publication ID: 27714

Stanley Langevin, Todd Lane, Pamela Lane, Zachary Bent, Owen Solberg, Deanna Curtis, Kelly Williams, Steven Branda, Kamlesh Patel, Joseph Schoeniger, (2012). cDNA normalization by hydroxyapatite chromatography to enhance transcriptome diversity in RNA-Seq Applications Nucleic Acid Research https://www.osti.gov/biblio/1078779 Publication ID: 26144

Susan Altman, Howland Jones, Steven Branda, Matthew Kirk, Christopher Marry, (2012). Linking ceragenins to water-treatment membranes to minimize biofouling https://doi.org/10.2172/1034896 Publication ID: 25840

Mais Jebrail, Anupama Sinha, Hanyoup Kim, Steven Branda, (2011). Diagnostic Platform For Rapid Identification and Virulence Characterization of Infectious Pathogens Based on Host Response https://www.osti.gov/servlets/purl/1143887 Publication ID: 25610

Stanley Mrowka, Steven Branda, (2011). Serum peptide enrichment using restricted access media in combination with proteolysis of high-abundance high molecular weight proteins Proteomics https://www.osti.gov/biblio/1106200 Publication ID: 24520

Carlee Ashley, Oscar Negrete, Gregory Sommer, Steven Branda, (2011). Engineered Virus-Like Particles for Pathogen Detection Vaccination and Targeted Delivery of Therapeutics https://www.osti.gov/servlets/purl/1662129 Publication ID: 24171

Gabriela Chirica, Joseph Schoeniger, Steven Branda, Steven Branda, Daniel Throckmorton, Stanley Mrowka, (2011). Search for Anthrax Biomarkers in Mouse Serum using a Mesofluidic Automated Processing Platform https://www.osti.gov/servlets/purl/1106271 Publication ID: 22555

Susan Rempe, Carol Kozina, Seema Singh, Steven Branda, Howland Jones, Michael Sinclair, (2011). Environmental perturbations to fluorescent proteins allow unambiguous discrimination of constructs with identical sequence Physical Biology https://www.osti.gov/biblio/1109408 Publication ID: 21560

Geun-Cheol Gil, Daniel Throckmorton, J. Brennan, Joseph Schoeniger, Victoria VanderNoot, Julia Fruetel, Steven Branda, (2010). Modular Automated Processing System (MAPS) for analysis of biological samples https://www.osti.gov/biblio/1030264 Publication ID: 20530

Daniel Throckmorton, J. Brennan, Steven Branda, Joseph Schoeniger, Gabriela Chirica, (2010). Modular Automated Processing System (MAPS) for High-Throughput Automated Serum Processing https://www.osti.gov/servlets/purl/1141658 Publication ID: 20429

Steven Branda, (2010). Host-Pathogen Molecular Biology https://www.osti.gov/servlets/purl/1675171 Publication ID: 20084

Darryl Sasaki, Julia Wang, Carl Hayden, Jeanne Stachowiak, Steven Branda, George Bachand, Robert Meagher, Mark Stevens, David Robinson, Frank Zendejas, (2010). Biomolecular transport and separation in nanotubular networks https://doi.org/10.2172/1008147 Publication ID: 19940

Michael Kent, Steven Branda, Carl Hayden, Darryl Sasaki, Kenneth Sale, (2010). Initiation of the TLR4 signal transduction network : deeper understanding for better therapeutics https://doi.org/10.2172/1008139 Publication ID: 20223

Steven Branda, (2010). Daniel Lozano DHS Paper – Final https://www.osti.gov/servlets/purl/1675343 Publication ID: 19370

Steven Branda, (2010). DHS-essay_Sergio LozanoFINAL https://www.osti.gov/servlets/purl/1675344 Publication ID: 19372

Milind Misra, Kamlesh Patel, Julia Kaiser, Robert Meagher, Steven Branda, Joseph Schoeniger, (2009). Host suppression and bioinformatics for sequence-based characterization of unknown pathogens https://doi.org/10.2172/1000286 Publication ID: 16978

Todd Lane, Pamela Lane, Steven Branda, Victoria VanderNoot, (2008). Rapid assessment of spore viability through on-chip gel electrophoresis of germination proteins Proposed for publication in Analytical Chemistry. https://www.osti.gov/biblio/966246 Publication ID: 15350

Steven Branda, Jens Poschet, Elizabeth Carles, Bryan Carson, Jaclyn Murton, Anup Singh, (2008). Characterization of the TLR4-Mediated Response to Y. pestis and E. coli LPS in Murine Macrophages https://www.osti.gov/servlets/purl/1142652 Publication ID: 14878

Steven Branda, Catherine Branda, Elizabeth Carles, Bryan Carson, David Haaland, Jaclyn Murton, Anup Singh, (2008). Copy of Transcription Factor Translocation in Response to Different Biothreat Agents LPS Chemotypes in Macrophages https://www.osti.gov/servlets/purl/1145520 Publication ID: 13169

Victoria VanderNoot, Todd Lane, Steven Branda, (2006). Small acid soluble proteins for rapid spore identification https://doi.org/10.2172/984135 Publication ID: 8613

Victoria VanderNoot, Steven Branda, Sara Gaucher, Todd Lane, (2005). Rapid onsite assessment of spore viability https://doi.org/10.2172/876524 Publication ID: 6243

Steven Branda, (2005). The master regulator for biofilm formation in Bacillus subtilis governs the expression of an operon encoding secreted proteins required for the assembly of complex multicellular communities Proposed for publication in Molecular Microbiology. https://www.osti.gov/biblio/972489 Publication ID: 5928

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Awards, Honors, and Memberships

NIH Study Section: NIAID Special Emphasis Panel “NIH Support for Conferences and Scientific Meetings R13/U13” (April 2014)
NIH Study Section: NIAID Special Emphasis Panel “Partnerships for Interventions to Treat Chronic, Persistent, and Latent Infections R21/R33″(November 2012)
NIH Study Section: NIAID Special Emphasis Panel “Biofilm P01” (January 2009) Postdoctoral Fellowship, Charles A. King Trust (2003-2005)
Postdoctoral Fellowship, American Cancer Society (2000-2003)
Postdoctoral Fellowship, Howard Hughes Medical Institute (1999-2000)
Predoctoral Fellowship, National Institutes of Health (1994-1997)
Sigma Xi, The Scientific Research Society (1991)
Distinction in Course, Swarthmore College (1991)

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